Beta Galactosidase

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Senescence-associated beta-galactosidase ( SA-β-gal or SABG ) is a hypothetical hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides only in senescent cells . Senescence-associated beta-galactosidase, along with p16 Ink4A , is regarded to be a biomarker of cellular senescence . [1]

The ONPG test can be used to: 1 Differentiate late lactose fermenters (P- G+) from non-lactose fermenters (P- G-). (a) Citrobacter (+) and Salmonella arizonae (+) from Salmonella (-). (b) Escherichia coli (+) from Shigella sonnei (-). 2 Aid in species differentiation. (a) Burkholderia cepacia (+) and Stenotrophomonas maltophila (+) from other Pseudomonas species (-). (b) Neisseria lactamica (+) from other Neisseria species (-).

First, to confirm overexpression of β-galactosidase, we examined endogenous β-galactosidase activity in several metastatic ovarian cancer cell lines established from human ovarian cancer patients (SHIN3 (ref. 17 ), SKOV3 (ref. 18 ), OVCAR3 (ref. 19 ), OVCAR4, OVCAR5, OVCAR8 and OVK18 (ref. 20 )). High levels of β-galactosidase activity were detected in these cell lines compared with that in a non-transformed human cell line, HUVEC (human umbilical vein endothelial cells; Fig. 1a ). This result suggested that β-galactosidase is a promising target for cancer imaging ( Fig. 1b ).

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  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase
  • Beta Galactosidase